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siRNA targeted knockdown For siRNA knockdown samples were transfected with 1 pmol siRNA together with 0.3 L Lipofectamine (Thermo Fisher Scientific, Waltham, MA) per 200 L and incubated for 24 h in media

However, the corresponding Phe22 residue in OsDHAR is located too far away from the H-site bound GSH (distance between the NH of Phe22 and S of GSH was 6.5 ) for an interaction to occur, even after the conformational change 20